Search results for "Heat inactivation"

showing 2 items of 2 documents

MboII endonuclease heat inactivation before agarose gel electrophoresis to prevent artifactual bands in restriction patterns

1999

Gel electrophoresisDNA BacterialElectrophoresis Agar GelProtein DenaturationSettore MED/07 - Microbiologia E Microbiologia ClinicaHot TemperaturebiologyMolecular biologyGeneral Biochemistry Genetics and Molecular BiologyRestriction fragmentHeat inactivationElectrophoresischemistry.chemical_compoundRestriction enzymeBiochemistrychemistryAgarose gel electrophoresisEnzyme Stabilitybiology.proteinEscherichia coliDeoxyribonucleases Type II Site-SpecificMboII endonucleaseDNAPolymorphism Restriction Fragment LengthBiotechnology
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Heat inactivation of fetal bovine serum increases protein contamination of extracellular vesicles

2022

Introduction: Extracellular vesicles (EVs) released in cell cultures are influenced by the cell culture conditions, such as the use of fetal bovine serum (FBS). FBS contains EVs and it is usually depleted of EVs by ultracentrifugation (UC) and/or heat inactivated (HI). Several studies have evaluated the effect of different UC protocols for FBS by evaluating both cells and EVs. However, less is known about the effect of HI on the cells and the released EVs. The aim of this study was therefore to evaluate the effect of HI on EV purity. Methods: To determine the effect of heat inactivation, three different protocols were applied based on different combinations of: 1) UC at 118,000 × g for 18h …

Heat inactivationFetal bovine serumExtracellular vesicles
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